Fig. 4

ALES was strongly and diffusely positive for cytokeratin AE1/AE3 (A, 10x), p40 (B, 10x), CK5/6 (C, 10x), membranous CD99 (D, 10x), and NKX2.2 (F, 10x), with varying degrees of nuclear positivity for FLI-1(E, 10x). ALES also demonstrated EWSR1-FLI1 translocations (G) without EWSRI-WT1 gene fusions (H) on fluorescent in-situ hybridization