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Fig. 3 | Diagnostic Pathology

Fig. 3

From: PSAT1 is upregulated by METTL3 to attenuate high glucose-induced retinal pigment epithelial cell apoptosis and oxidative stress

Fig. 3

Effects of PSAT1 on HG-induced ARPE-19 cell injury. (A and B) RT-qPCR assay and Western blot assay were used to detect PSAT1 expression in ARPE-19 cells transfected with Vector or OE-PSAT1. (C-H) ARPE-19 cells were treated with NG, HG + Vector, or HG + OE-PSAT1. (C) Cell viability was assessed in treated ARPE-19 cells using CCK-8 assay. (D-F) ROS, MDA, and GSH-Px levels were measured using Commercial kits in treated ARPE-19 cells. (G) Cell apoptosis was examined using TUNEL assay in treated ARPE-19 cells. (H) Bcl-2 and Bax protein levels in treated ARPE-19 cells were detected using western blot. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3

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Diagnostic Pathology

ISSN: 1746-1596

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