Detection of genetic mutations in 855 cases of papillary thyroid carcinoma by next generation sequencing and its clinicopathological features

Table 1 Primer sequence of PCR

Gene		Primer sequence
BRAF V600E	Forward	5’-TCTGTAGCAGCCCTCAGTAGCGAAGCAGTGATTTTGGTCTAGCTACAGA-3’
	Reverse	5’-AGCCCTCAGTAGCGAAGCAACTCAGCAGCATCTCAGG − 3’
	Probe	5’-TTCAAACCATCAGTTTGAACAGTTGTCTGGATCAACTG-3’
Internal Reference	Forward	5’-GTACCTGCAAGGTGTGGAGTTAC-3’
	Reverse	5’-GCCATCCTGAATTCTGTAAACAGC-3’
	Probe	5’-AGACCTCTCATCATCAGTGC-3’

ISSN: 1746-1596