Fig. 1

ADAMTS-1 immunostaining in odontogenic keratocyst (OKC) and dental follicle (DF). ADAMTS-1 staining was diffuse in the epithelial layers of OKC, with a predominantly cytoplasmic localization (A, arrows). DF also expressed ADAMTS-1, with predominantly cytoplasmic staining (B, arrows). There was no significant difference in the area of ADAMTS-1 staining between OKC and DF (C). However, ADAMTS-1 immunostaining in OKC was more pronounced in epithelial cells than in the capsule (D). ADAMTS-1 gene expression was reduced in OKC compared with DF, although without a statistically significant difference. RT-PCR analysis was performed using the Pfaffl method to calculate relative mRNA levels normalized by β-actin (E) or GAPDH (F). Significance: ∗∗∗p < 0.001. Scale bar: 20 μm