Fig. 3

Brevican immunostaining in odontogenic keratocyst (OKC) and dental follicle (DF). Brevican staining was diffuse in the epithelial layers of OKC, with predominantly cytoplasmic localization (A, arrows). DF also expressed brevican, with predominantly cytoplasmic staining (B, arrows). There was no significant difference in brevican labeling area between OKC and DF (C). However, brevican immunostaining in OKC was more pronounced in the epithelial cells than in the capsule (D). There was no statistically significant difference in brevican gene expression between OKC and DF, as determined by RT-PCR analysis using the Pfaffl method to calculate relative mRNA levels normalized by β-actin (E) or GAPDH (F). Significance: ∗∗∗p < 0.001. Scale bars: 20 μm and 50 μm