Fig. 4

Versican immunostaining in odontogenic keratocyst (OKC) and dental follicle (DF). Versican staining was predominantly cytoplasmic in the basal and parabasal layers of OKC epithelial cells (A, arrows). DF showed weak versican labeling, with predominantly cytoplasmic localization (B, arrows). The versican labeling area indicated higher expression in the epithelial cells of OKC compared to DF (C). Moreover, versican immunostaining in OKC was more prominent in the epithelial cells than in the capsule (D). There was no statistically significant difference in versican gene expression between OKC and DF, as determined by RT-PCR analysis using the Pfaffl method to calculate relative mRNA levels normalized by β-actin (E) or GAPDH (F). Significance: *p < 0.05, ∗∗∗p < 0.001. Scale bar: 20 μm