Fig. 3

The immunostaining characteristics of S100A10 protein in cases of papillary thyroid carcinoma (PTC) with and without TERTp mutation. (A-C) showed a representative case of PTC with the wildtype genotype. The tumor’s morphology was displayed in figure A (H&E, 200×). The tumor cells showed a weak immunostaining with S100A10 (B, 200×). No TERTp mutations were identified in this case. The DNA sequence encompassing the c.-124 and c.-112 sites was visualized in figure C. (D-F) demonstrated a representative case with the C216T mutation. The tumor’s morphology was displayed in figure D (H&E, 200×). The tumor cells showed a weak immunostaining with S100A10 (E, 200×). The C216T mutation, located at position c.-112, was detected in this case, and a de novo TCTC motif was generated (F). (G-I) presented a representative case with the C228T mutation. The tumor’s morphology was displayed in figure G (H&E, 200×). The tumor cells exhibited strong immunostaining with S100A10 (H, 200×). The C228T mutation, located at position c.-124, was detected in this case, and a de novo CCTT motif was generated (I)