Fig. 4

In this slide, both low-grade PanIN, and invasive ductal carcinoma are present in the context of pancreatic ductal carcinoma (A, H&E, magnification x100; B, ACSL4, magnification x100). The low-grade PanIN exhibits ACSL4 low expression, graded as 1 or 2 (C, H&E, magnification x400; D, ACSL4, magnification x400). In contrast, invasive ductal carcinoma exhibit high ACSL4 expression (E, H&E, magnification x400; F, ACSL4, magnification x400). Even when low-grade PanIN and invasive PDAC were found in the same specimen, staining differences were typically clear, with low-grade PanIN being negative and invasive PDAC being positive